Journal: PLoS ONE

Article Title: Meta-Analysis of Placental Transcriptome Data Identifies a Novel Molecular Pathway Related to Preeclampsia

doi: 10.1371/journal.pone.0132468

Figure Lengend Snippet: Datasets included in the meta-analysis.

Article Snippet: Jarvenpaa [ ] , 3 , 2 , Affymetrix HG U133 plus 2.0.

Techniques: Microarray

Journal: PLoS ONE

Article Title: Meta-Analysis of Placental Transcriptome Data Identifies a Novel Molecular Pathway Related to Preeclampsia

doi: 10.1371/journal.pone.0132468

Figure Lengend Snippet: Studies excluded in the meta-analysis.

Article Snippet: Jarvenpaa [ ] , 3 , 2 , Affymetrix HG U133 plus 2.0.

Techniques:

Journal: American Journal of Respiratory and Critical Care Medicine

Article Title: Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium

doi: 10.1164/rccm.202003-0541OC

Figure Lengend Snippet: Expression of ACE2 (angiotensin-converting enzyme 2) in the human airway epithelium of healthy nonsmokers. ( A and B ) Expression level is presented as relative gene expression compared with all other genes on the array. See the online supplement for details on normalization. ( A ) Comparison of ACE2 expression in trachea epithelium, large airway epithelium, and small airway epithelium (SAE). Quantification was by Affymetrix HG-U133 Plus 2.0 microarrays. The data were generated from the data sets of Gene Expression Omnibus accession numbers 13933, 10135, and 11784 ( , , ) and were compared using a two-way ANOVA (sex was identified as a source of variation). ( B ) ACE2 expression during in vitro differentiation of airway epithelium derived from primary tracheal basal cells (BCs). RNA was collected by brushing from freshly isolated, purified tracheal BCs and from cells derived from the BCs on an air–liquid interface culture at the initiation of the culture (Day 0) and at Days 7–28 of culture. ACE2 levels (determined by Affymetrix HG-U133 Plus 2.0 microarrays) increased as BCs differentiated into airway epithelial cells ( ACE2 levels at Day 28 compared with Day 0, P < 10 −5 ). ( C ) Single-cell 10x analysis of ACE2 expression in the different cell populations from the normal SAE of healthy nonsmokers. All the major cell types express ACE2 , including basal, intermediate, club, mucus, and ciliated cells. Each data point represents a single cell. ACE2 was detected in a minority of epithelial cells from each cluster (detected in 1.2% of BCs, 2.6% of intermediate cells, 1.7% of club cells, 2.4% of mucus cells, and 1.0% of ciliated cells). These values are useful for comparison among the epithelial cell types but underestimate the actual percentage of cells expressing the gene . See the online supplement for markers used to define each cell type and for details on the calculation of scaled unique molecular identifiers and transformation for data presentation. *** P < 0.001. ALI = air–liquid interface; LAE = large airway epithelium; NS = nonsignificant; UMI = unique molecular identifiers.

Article Snippet: ACE2 expression was higher in the SAE of smokers than nonsmokers (Affymetrix HG-U133 Plus 2.0 microarray; P < 10 −5 ; ).

Techniques: Expressing, Generated, In Vitro, Derivative Assay, Isolation, Purification, Transformation Assay

Journal: American Journal of Respiratory and Critical Care Medicine

Article Title: Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium

doi: 10.1164/rccm.202003-0541OC

Figure Lengend Snippet: Effect of smoking and sex on ACE2 (angiotensin-converting enzyme 2) expression in the small airway epithelium. ( A and B ) Expression level is presented as relative gene expression compared with all other genes on the array. See the online supplement for details on normalization. ( A ) Healthy smokers (gray symbols) versus nonsmokers (white symbols), with male and female sexes combined (Affymetrix HG-U133 Plus 2.0 microarrays). The data were generated from the data set of Tilley and colleagues , Gene Expression Omnibus accession number 11784. ( B ) Male sex versus female sex for smokers versus nonsmokers (Affymetrix HG-U133 Plus 2.0 microarrays), using the same data set as in A . ( C ) Smokers versus nonsmokers, male and female sexes combined, RNA sequencing (RNA-seq) (HiSeq 2500; Illumina). ( D ) Male versus female sex for smokers versus nonsmokers (RNA-seq), using the same data set as in C . A two-way ANOVA (sex was identified as a source of variation) was used for analysis. * P < 0.05, ** P < 0.01, and *** P < 0.001. FPKM = fragments per kilobase of exon per million fragments sequenced; NS = nonsignificant; SAE = small airway epithelium.

Article Snippet: ACE2 expression was higher in the SAE of smokers than nonsmokers (Affymetrix HG-U133 Plus 2.0 microarray; P < 10 −5 ; ).

Techniques: Expressing, Generated, RNA Sequencing Assay